From bc30be8623dc1efb378b31db42fc0a44d3e0b8d9 Mon Sep 17 00:00:00 2001 From: "W. Trevor King" Date: Thu, 27 Jun 2013 17:03:30 -0400 Subject: [PATCH] Distinguish between glutamine and glutamic acid This is important ;). Thanks, Prof. Cruz. --- src/apparatus/polymer-synthesis.tex | 3 ++- src/future/salt.tex | 6 +++++- 2 files changed, 7 insertions(+), 2 deletions(-) diff --git a/src/apparatus/polymer-synthesis.tex b/src/apparatus/polymer-synthesis.tex index a2795c0..31bbf3b 100644 --- a/src/apparatus/polymer-synthesis.tex +++ b/src/apparatus/polymer-synthesis.tex @@ -123,7 +123,8 @@ attracted to the metal ion. \nomenclature[text ]{Asn}{Asparagine, an amino acid.} \nomenclature[text ]{Asp}{Aspartic acid, an amino acid.} \nomenclature[text ]{Cys}{Cystine, an amino acid.} -\nomenclature[text ]{Glu}{Glutamine, an amino acid.} +\nomenclature[text ]{Glu}{Glutamic acid, an amino acid.} +\nomenclature[text ]{Gln}{Glutamine, an amino acid.} \nomenclature[text ]{Gly}{Glycine, an amino acid.} \nomenclature[text ]{His}{Histidine, an amino acid.} \nomenclature[text ]{Ile}{Isoleucine, an amino acid.} diff --git a/src/future/salt.tex b/src/future/salt.tex index 8bbe12f..c7df0fb 100644 --- a/src/future/salt.tex +++ b/src/future/salt.tex @@ -6,7 +6,11 @@ strength of the buffer did significantly decrease the unfolding force (folding stability) of I27. For labs with strong gene-splicing capability, it would be interesting to replace the glutamic acids involved in the major bonding (\cref{fig:I27:H-bonds}) with -alternative groups to gauge the specificity of the effect. +alternative groups to gauge the specificity of the effect. For +example, glutamine is identical to glutamic acid, except that it has a +hydroxyl group (OH) in the side-chain where glutamic acid has an +amine group (NH\textsubscript{2}). This gives glutamine and glutamic +acid similar steric properties, but very different chemistry. While the statistics are strong for the two concentrations we tested (standard PBS and PBS with an additional $0.5\U{M}$ \CaCl), it would -- 2.26.2