X-Git-Url: http://git.tremily.us/?p=hooke.git;a=blobdiff_plain;f=doc%2Fanalysis.txt;fp=doc%2Fanalysis.txt;h=1286393a53f575c0a517dc5232d20633c5c4fea6;hp=0000000000000000000000000000000000000000;hb=30d5d93368e3170161f6a9d4f94dc8f0f958277a;hpb=64dbf40808667c1cafdfebce9f67fdacb30d7cf4 diff --git a/doc/analysis.txt b/doc/analysis.txt new file mode 100644 index 0000000..1286393 --- /dev/null +++ b/doc/analysis.txt @@ -0,0 +1,85 @@ +****************** +Analysis templates +****************** + +.. toctree:: + :maxdepth: 2 + +Overview +======== + +Hooke is a large, flexible beast, and the number of available commands +can be intimidating. In this section, we'll lay out the procedure for +analyzing a number of common experiment types. The experiment types +are arranged in the order that they were developed, citating the first +known paper in each class. If we've got a citation wrong, please +submit a bug report (:doc:`bugs`). + + +Velocity clamp force spectroscopy +================================= + +Developed by Rief et al. [#rief1997]_, velocity clamp spectroscopy +measures domain unfolding forces as the tip is moved away from the +surface at a constant velocity. + +.. todo:: Velocity clamp example plot. + +Basic analysis is something like:: + + hooke> zero_surface_contact_point --block retract + hooke> flat_filter_peaks --block retract --min_points 1 + hooke> zero_surface_contact_point --block retract + ... --ignore_after_last_peak_info_name 'flat filter peaks' + hooke> convert_distance_to_force --block retract + ... --deflection_column 'surface deflection (m)' + hooke> remove_cantilever_from_extension --block retract + hooke> flat_peaks_to_polymer_peaks --block retract + hooke> polymer_fit_peaks --block retract + hooke> block_info --block retract --output data.yaml name 'polymer peak [0-9]*' + +The unfolding forces should be grouped by pull velocity, and the resulting +distributions can be fit to specific unfolding models by comparison with +monte-carlo simulations (see, for example, sawsim_). + +.. [#rief1997] M. Rief, M. Gautel, F. Oesterhelt, J.M. Fernandez, + H.E. Gaub. + "Reversible Unfolding of Individual Titin Immunoglobulin Domains by AFM." + Science, 1997. + doi: `10.1126/science.276.5315.1109 `_ + +.. _sawsim: http://www.physics.drexel.edu/~wking/sawsim/ + +Force clamp spectroscopy +======================== + +Developed by Fernandez and Li [#fernandez2004]_, force clamp spectroscopy +measures the lifetime of a domain's folded state while subject to a constant +tension. + +.. todo:: Force clamp example plot. + +.. todo:: Force clamp example analysis. + +.. [#fernandez2004] J.M. Fernandez, H. Li. + "Force-Clamp Spectroscopy Monitors the Folding Trajectory of a + Single Protein." + Science, 2004. + doi: `10.1126/science.1092497 `_ + +Two-color fluorescence coincidence spectroscopy +=============================================== + +Developed by Clarke et al. [#clarke2007]_,... + +.. todo:: TCCS explanation. + +.. todo:: TCCS example plot. + +.. todo:: TCCS example analysis. + +.. [#clarke2007] R.W. Clarke, A. Orte, D. Klenerman. + "Optimized Threshold Selection for Single-Molecule Two-Color + Fluorescence Coincidence Spectroscopy." + Anal. Chem., 2007. + doi: `10.1021/ac062188w `_