1 # Copyright (C) 2010 W. Trevor King <wking@drexel.edu>
3 # This file is part of Hooke.
5 # Hooke is free software: you can redistribute it and/or
6 # modify it under the terms of the GNU Lesser General Public
7 # License as published by the Free Software Foundation, either
8 # version 3 of the License, or (at your option) any later version.
10 # Hooke is distributed in the hope that it will be useful,
11 # but WITHOUT ANY WARRANTY; without even the implied warranty of
12 # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
13 # GNU Lesser General Public License for more details.
15 # You should have received a copy of the GNU Lesser General Public
16 # License along with Hooke. If not, see
17 # <http://www.gnu.org/licenses/>.
19 """Driver for W. Trevor King's velocity clamp data format.
21 See my related projects:
23 * http://www.physics.drexel.edu/~wking/code/git/git.php?p=unfold_protein.git
24 * http://www.physics.drexel.edu/~wking/code/git/git.php?p=calibrate_cantilever.git
25 * http://www.physics.drexel.edu/~wking/code/git/git.php?p=data_logger.git
26 * http://www.physics.drexel.edu/~wking/code/git/git.php?p=piezo.git
27 * http://www.physics.drexel.edu/~wking/code/git/git.php?p=pycomedi.git
29 And the deprecated projects (which Hooke replaces):
31 * http://www.physics.drexel.edu/~wking/code/git/git.php?p=scale_unfold.git
32 * http://www.physics.drexel.edu/~wking/code/git/git.php?p=sawmodel.git
44 from .. import curve as curve
45 from .. import experiment as experiment
46 from ..config import Setting
47 from . import Driver as Driver
50 import calibcant.config
51 calibcant_dir = calibcant.config.LOG_DIR
61 class WTKDriver (Driver):
62 """Handle W. Trevor King's data_logger data format.
65 super(WTKDriver, self).__init__(name='wtk')
67 def default_settings(self):
69 Setting(section=self.setting_section, help=self.__doc__),
70 Setting(section=self.setting_section,
71 option='cantilever calibration directory',
73 help='Set the directory where cantilever calibration data is stored'),
76 def is_me(self, path):
77 if not path.endswith('_unfold'):
79 for p in self._paths(path):
80 if not os.path.isfile(p):
84 def read(self, path, info=None):
85 approach_path,retract_path,param_path = self._paths(path)
87 unlabeled_approach_data = numpy.loadtxt(
88 approach_path, dtype=numpy.uint16)
89 unlabeled_retract_data = numpy.loadtxt(
90 retract_path, dtype=numpy.uint16)
91 params = self._read_params(param_path)
92 params = self._translate_params(params)
94 # move data into Data blocks.
95 approach = self._scale_block(
96 unlabeled_approach_data, params, 'approach')
97 retract = self._scale_block(
98 unlabeled_retract_data, params, 'retract')
99 info = {'filetype':self.name, 'experiment':experiment.VelocityClamp}
100 return ([approach, retract], info)
102 def _paths(self, path):
103 return (path+'_approach', path, path+'_param')
105 def _read_params(self, param_path):
107 for line in file(param_path):
108 ldata = [x.strip() for x in line.split(':', 1)]
109 if ldata[0] == 'Data fields':
110 params['columns'] = ldata[1].split()
111 elif len(ldata) == 2:
112 argwords = ldata[1].split()
113 if ldata[0] == 'Time' or len(argwords) != 1:
114 params[ldata[0]] = ldata[1]
115 else: # single word probably a number
116 params[ldata[0]] = float(ldata[1])
118 pass # ignore comment lines
121 def _translate_params(self, params):
122 ret = {'raw info':params,}
124 t = params['Time'] # 20100504135849
125 ret['time'] = self._time_from_localtime_string(t)
127 assert params['columns'] == ['Z_piezo_out', 'Deflection_in', 'Z_piezo_in'], \
128 'Unexpected columns: %s' % ret['columns']
129 ret['columns'] = ['z piezo (m)', 'deflection (m)', ]
131 calibcant_file = self._find_previous_cantilever_calibration_file(
133 calibcant_info = self._read_cantilever_calibration_file(calibcant_file)
134 ret['raw spring constant'] = calibcant_info
135 ret['spring constant (N/m)'] = calibcant_info['Cantilever k (N/m)']
136 ret['deflection sensitivity (m/V)'] = \
137 1.0/numpy.sqrt(calibcant_info['photoSensitivity**2 (V/nm)**2'])
139 # (32768 bits = 2**15 bits = 10 Volts)
140 ret['deflection sensitivity (V/bit)'] = 1.0/3276.8
141 ret['deflection range (V)'] = 20.0
142 ret['deflection offset (V)'] = 10.0 # assumes raw data is unsigned
144 ret['z piezo sensitivity (V/bit)'] = 1.0/3276.8 # output volts / bit
145 ret['z piezo range (V)'] = 20.0 # output volts
146 ret['z piezo offset (V)'] = 10.0 # output volts, assumes unsigned
147 ret['z piezo gain'] = \
148 params['Z piezo gain (Vp/Vo)'] # piezo volts / output volt
149 ret['z piezo sensitivity (m/V)'] = \
150 params['Z piezo sensitivity (nm/Vp)'] * 1e-9 # m / piezo volts
154 def _scale_block(self, data, info, name):
155 """Convert the block from its native format to a `numpy.float`
159 shape=(data.shape[0], 2),
161 info=copy.deepcopy(info)
163 ret.info['name'] = name
164 ret.info['raw data'] = data # store the raw data
165 ret.info['columns'] = ['z piezo (m)', 'deflection (m)']
168 # approach data does not have a Z_piezo_in column as of unfold v0.0.
169 z_rcol = info['raw info']['columns'].index('Z_piezo_out')
170 d_rcol = info['raw info']['columns'].index('Deflection_in')
172 # scaled column indices
173 z_scol = ret.info['columns'].index('z piezo (m)')
174 d_scol = ret.info['columns'].index('deflection (m)')
176 # Leading '-' because increasing voltage extends the piezo,
177 # moving the tip towards the surface (positive indentation),
178 # but it makes more sense to me to have it increase away from
179 # the surface (positive separation).
181 (data[:,z_rcol].astype(ret.dtype)
182 * info['z piezo sensitivity (V/bit)']
183 - info['z piezo offset (V)'])
184 * info['z piezo gain']
185 * info['z piezo sensitivity (m/V)']
188 # Leading '-' because deflection voltage increases as the tip
189 # moves away from the surface, but it makes more sense to me
190 # to have it increase as it moves toward the surface (positive
191 # tension on the protein chain).
194 * info['deflection sensitivity (V/bit)']
195 - info['deflection offset (V)'])
196 * info['deflection sensitivity (m/V)']
201 def _list_re_search(self, list, regexp):
202 "Return list entries matching re"
203 reg = re.compile(regexp)
210 def _list_cantilever_calibration_files(self, timestamp=None, basedir=None):
211 if timestamp == None:
212 timestamp = time.time()
214 basedir = self.config['cantilever calibration directory']
215 YYYYMMDD = time.strftime("%Y%m%d", time.localtime(timestamp))
216 dir = os.path.join(data_logger.normalize_logdir(basedir), YYYYMMDD)
217 if not os.path.exists(dir):
219 all_calibfiles = os.listdir(dir) # everything in the directory
220 #regexp = "%s.*_analysis_text " % (YYYYMMDD)
221 regexp = ".*_analysis_text"
222 calibfiles = self._list_re_search(all_calibfiles, regexp)
223 paths = [os.path.join(dir, cf) for cf in calibfiles]
226 def _calibfile_timestamp(self, path):
227 filename = os.path.basename(path)
228 YYYYMMDDHHMMSS = filename[0:14]
229 return self._time_from_localtime_string(YYYYMMDDHHMMSS)
231 def _find_previous_cantilever_calibration_file(
232 self, timestamp=None, basedir=None, previous_days=1):
234 If timestamp == None, uses current time.
235 Warning : brittle! depends on the default data_logger.py save filesystem.
236 Renaming files or moving directories will break me.
238 #if FORCED_CANTILEVER_CALIBRATION_FILE != None:
239 # return FORCED_CANTILEVER_CALIBRATION_FILE
241 for i in range(previous_days+1):
242 calibfiles.extend(self._list_cantilever_calibration_files(
243 timestamp-24*3600*i,basedir=basedir))
244 assert len(calibfiles) > 0 , \
245 "No calibration files in that day range in directory '%s'" % basedir
247 for i in range(len(calibfiles)):
248 if self._calibfile_timestamp(calibfiles[i]) > timestamp:
253 def _read_cantilever_calibration_file(self, filename):
254 ret = {'file': filename}
255 line_re = re.compile('(.*) *: (.*) [+]/- (.*) \((.*)\)\n')
256 for line in file(filename):
257 match = line_re.match(line)
260 'Invalid cantilever calibration line in %s: %s'
262 key,value,std_dev,rel_err = [
263 x.strip() for x in match.group(*range(1,5))]
264 if key == 'Variable (units)':
265 continue # ignore the header line
267 std_dev = float(std_dev)
268 # Handle older calibcant output.
270 key = 'Cantilever k (N/m)'
271 elif key == 'a**2 (V/nm)**2':
272 key = 'photoSensitivity**2 (V/nm)**2'
274 ret[key + ' (std. dev.)'] = std_dev
277 def _time_from_localtime_string(self, timestring, format="%Y%m%d%H%M%S"):
279 >>> print time.tzname
282 >>> d._time_from_localtime_string("19700101", format="%Y%m%d")/3600.0
284 >>> d._time_from_localtime_string("20081231", format="%Y%m%d")
286 >>> d._time_from_localtime_string("20090101", format="%Y%m%d")
289 timestruct = time.strptime(timestring, format)
290 timestamp = calendar.timegm(timestruct)
291 # Date strings are in localtime, so what we really want is
292 # .timelocale, but that doesn't exist. Workaround to convert
294 timestamp += time.timezone # assume no Daylight Savings Time (DST)
295 if bool(time.localtime(timestamp).tm_isdst) == True:
296 timestamp -= time.timezone - time.altzone # correct if DST
297 assert time.strftime(format, time.localtime(timestamp)) == timestring, "error in time_from_localtime_string, %s != %s" % (time.strftime(format, time.localtime(timestamp)), timestring)