From d815f72101bc03c4f780a5cadc685c50dc46fbaf Mon Sep 17 00:00:00 2001 From: "W. Trevor King" Date: Wed, 31 Aug 2011 15:08:24 -0400 Subject: [PATCH] Fix BamI sticky end in PstI+BamHI step of I27-synthesis post. Also fix Qiagen linker site link. --- posts/I27-synthesis.mdwn | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/posts/I27-synthesis.mdwn b/posts/I27-synthesis.mdwn index 236a996..e5eb42e 100644 --- a/posts/I27-synthesis.mdwn +++ b/posts/I27-synthesis.mdwn @@ -204,7 +204,7 @@ The single-SP plasmid, pSP4-1, is split in two parallel reactions. #### PstI + BamHI G...AGATCT-SP-G - ACGTC...TCTAGA-SP-CCTAGG + ACGTC...TCTAGA-SP-CCTAG #### PstI + BglII @@ -282,7 +282,7 @@ vector", but I'm not sure why they would need a non-expression vector, as they don't reference cross-vector subcloning after inserting their I27 monomer into the plasmid. -From the [Qiagen site][pQE30], the section around the linker +From the [Qiagen site][pQE30-b], the section around the linker nucleotides 115 through 203 is: ,RGS-His epitope__________________. ,BamHI. -- 2.26.2