From caf7a4a6e4c300fc75d5252eb550d5bb017a4032 Mon Sep 17 00:00:00 2001 From: "W. Trevor King" Date: Thu, 18 Nov 2010 13:10:29 -0500 Subject: [PATCH] Add force spectroscopy and research posts. --- posts/Force_spectroscopy.mdwn | 41 ++++++++++++++++++ .../afm_protein_pulling_cartoon.jpg | Bin 0 -> 90593 bytes .../force_curve_regions.png | Bin 0 -> 19788 bytes .../force_curve_regions_cantilever.png | Bin 0 -> 22783 bytes posts/Gentoo_overlay.mdwn | 2 +- posts/Hooke.mdwn | 12 ++--- posts/Research.mdwn | 20 +++++++++ posts/sawsim.mdwn | 3 +- 8 files changed, 69 insertions(+), 9 deletions(-) create mode 100644 posts/Force_spectroscopy.mdwn create mode 100644 posts/Force_spectroscopy/afm_protein_pulling_cartoon.jpg create mode 100644 posts/Force_spectroscopy/force_curve_regions.png create mode 100644 posts/Force_spectroscopy/force_curve_regions_cantilever.png create mode 100644 posts/Research.mdwn diff --git a/posts/Force_spectroscopy.mdwn b/posts/Force_spectroscopy.mdwn new file mode 100644 index 0000000..afa48f7 --- /dev/null +++ b/posts/Force_spectroscopy.mdwn @@ -0,0 +1,41 @@ +[Force spectroscopy][fs] is the process of extracting information +about the unfolding (or unbinding) characteristics of a protein (or +ligand-receptor pair) by measuring force vs. extension curves while +gradually ripping the protein (or pair) apart. Consider this cartoon +representation of the procedure + +[[!img afm_protein_pulling_cartoon.jpg class="scaled" + alt="Protein unfolding cartoon" + title="Protein unfolding cartoon"]] + +The AFM tip is pulling a protein chain away from the substrate, +causing one of the protein domains to uncoil. + +The procedure yields 'force curves' like this + +[[!img force_curve_regions.png class="scaled" + alt="Protein unfolding force curve" + title="Protein unfolding force curve"]] + +To interpret the force curve, let us examine it piece-by-piece as the +AFM tip gradually pulls away from the substrate. + +1. The linear 'contact' region demonstrates the Hooke's law behavior + of the AFM cantilever, with force ∝ displacement. +2. The high force 'bulge' linking the contact region to the sawtooth + comes from the AFM tip pulling free of the surface and associated + protein 'mat' (the cartoon being excessively pretty, and our sample + having too high a protein concentration :p). +3. The characteristic 'sawtooth' comes from several identical domains + unfolding one after the other. +4. After the last of the protein domains unfolds the protein snaps off + of the AFM tip (or the substrate), and the deflection of the + now-free cantilever ceases to depend on distance. + +[[!img force_curve_regions_cantilever.png class="scaled" + alt="Force curve cantilever positions" + title="Force curve cantilever positions"]] + +[fs]: http://en.wikipedia.org/wiki/Force_spectroscopy + +[[!tag tags/theory]] diff --git a/posts/Force_spectroscopy/afm_protein_pulling_cartoon.jpg b/posts/Force_spectroscopy/afm_protein_pulling_cartoon.jpg new file mode 100644 index 0000000000000000000000000000000000000000..babc3befdfbbce38d29e3d98cdc0c2f25be6ff3e GIT binary patch literal 90593 zcmeFZby!th*EhQ85Rev-?#4~C>F(}Ogw3YAK?xD*Zlp`31q4JSq&uVqM5I$vrOyKI z`+1+|{oWJzcdql#xm>vBT64@X#~5?WSbP0kV_wf(e+6)rg4bSEuzR8Z5u>_;H}FZ&TF z|J{B>gui7VFe3g-HZExA?V$jsH}|*K>;Lc}NCT&qhEI-h=W$5Alx3H z!8ZpII6#n5P|?saFz!UdjuWQhlG#W#K_WsfV%#DvYzvelkOrnm3YTKgd&uW#iQnp)h@Grj&3 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