From 3b3c945457b98745c1de9c5feb6e274d8500c31e Mon Sep 17 00:00:00 2001 From: "W. Trevor King" Date: Fri, 26 Jun 2009 14:55:20 -0400 Subject: [PATCH] Added README file. Also a bug/thoughts on packaging trouble (blocked by temperature). --- .../ebbf4721-8e0b-4d9f-a1e3-bbf1f26fbff9/body | 6 +++ .../values | 8 +++ .../values | 17 ++++++ .be/settings | 2 + .be/version | 1 + README | 52 +++++++++++++++++++ 6 files changed, 86 insertions(+) create mode 100644 .be/bugs/a2b804be-9892-452c-8c82-4b2cd245c5f3/comments/ebbf4721-8e0b-4d9f-a1e3-bbf1f26fbff9/body create mode 100644 .be/bugs/a2b804be-9892-452c-8c82-4b2cd245c5f3/comments/ebbf4721-8e0b-4d9f-a1e3-bbf1f26fbff9/values create mode 100644 .be/bugs/a2b804be-9892-452c-8c82-4b2cd245c5f3/values create mode 100644 .be/settings create mode 100644 .be/version create mode 100644 README diff --git a/.be/bugs/a2b804be-9892-452c-8c82-4b2cd245c5f3/comments/ebbf4721-8e0b-4d9f-a1e3-bbf1f26fbff9/body b/.be/bugs/a2b804be-9892-452c-8c82-4b2cd245c5f3/comments/ebbf4721-8e0b-4d9f-a1e3-bbf1f26fbff9/body new file mode 100644 index 0000000..3381a3e --- /dev/null +++ b/.be/bugs/a2b804be-9892-452c-8c82-4b2cd245c5f3/comments/ebbf4721-8e0b-4d9f-a1e3-bbf1f26fbff9/body @@ -0,0 +1,6 @@ +This is waiting while I figure out how to bundle temperature +appropriately. Currently my SWIG MelcorTempControl depends on my C +serial_io code, but there's no setup/distutils method of automatically +including this dependency information. Maybe I should just write an +ebuild? + diff --git a/.be/bugs/a2b804be-9892-452c-8c82-4b2cd245c5f3/comments/ebbf4721-8e0b-4d9f-a1e3-bbf1f26fbff9/values b/.be/bugs/a2b804be-9892-452c-8c82-4b2cd245c5f3/comments/ebbf4721-8e0b-4d9f-a1e3-bbf1f26fbff9/values new file mode 100644 index 0000000..8c94b78 --- /dev/null +++ b/.be/bugs/a2b804be-9892-452c-8c82-4b2cd245c5f3/comments/ebbf4721-8e0b-4d9f-a1e3-bbf1f26fbff9/values @@ -0,0 +1,8 @@ +Content-type: text/plain + + +Date: Fri, 26 Jun 2009 18:53:22 +0000 + + +From: W. Trevor King + diff --git a/.be/bugs/a2b804be-9892-452c-8c82-4b2cd245c5f3/values b/.be/bugs/a2b804be-9892-452c-8c82-4b2cd245c5f3/values new file mode 100644 index 0000000..0e52dac --- /dev/null +++ b/.be/bugs/a2b804be-9892-452c-8c82-4b2cd245c5f3/values @@ -0,0 +1,17 @@ +creator: W. Trevor King + + +reporter: W. Trevor King + + +severity: minor + + +status: open + + +summary: unfold.py not packaged + + +time: Fri, 26 Jun 2009 18:12:32 +0000 + diff --git a/.be/settings b/.be/settings new file mode 100644 index 0000000..739d28c --- /dev/null +++ b/.be/settings @@ -0,0 +1,2 @@ +rcs_name: git + diff --git a/.be/version b/.be/version new file mode 100644 index 0000000..990837e --- /dev/null +++ b/.be/version @@ -0,0 +1 @@ +Bugs Everywhere Tree 1 0 diff --git a/README b/README new file mode 100644 index 0000000..d96ea23 --- /dev/null +++ b/README @@ -0,0 +1,52 @@ +Unfolding experiment procedure: + +At the ipython command line (use -pylab option for easy plotting) +>>> import unfold +>>> u = unfold.unfold(controlTemp=False) + +Approach the surface +>>> u.stepApproach(setpoint=1.0) +Hold down during approach to keep O-ring from lifting the +head. Diagnose by watching under video-microscope: head movement +shifts picture. Alternatively, use the flexible S-crossectioned +"o-rings" or scrunchies to clamp head on tighter ;). + +Wiggle for interference just off the surface +>>> u.stepAway(); u.stepAway(); u.stepAway() +>>> import z_piezo_utils +>>> z_piezo_utils.wiggleForInterferenceMin(u.zp, wig_freq=1.0) + +Calibrate +>>> import calibcant.calibrate +>>> calibcant.config.DEFAULT_TEMP = 25 # degrees C +>>> calibcant.calibrate.calib(stepper=u.step, zpiezo=u.zp, push_depth=90) +push_depth is in nm. There are a host of other keyword arguments to +calib. See them all with +>>> calibcant.calibrate.calib._kwargs(calibcant.calibrate.calib) + +Tweak coarse surface position (these are stepper-backlash protected functions) +>>> u.stepCloser() +>>> u.stepAway() + +Unfold a protein +>>> u.unfold(rel_setpoint=2, nmDist=1400,...) + +There's also a convenience function `loop_rates` +>>> rates = [250, 500, 750, 1000] +>>> unfold.loop_rates(u, rates=rates, num_loops=10, die_file="~/die", + rel_setpoint=1, nmDist=800, sBindTime=2, ...) +which unfolds at each rate 10 times, unless ~/die exist, in which case +it stops nicely. rel_setpoint, nmDist, sBindTime, and other additional +options are just passed on to unfold.unfold(). + +Modules : +see unfold.png + + +Remember, +>>> help(whatever) +is your friend in Python, and +$ man whatever +and +$ whatever --help +are your friends in a shell. -- 2.26.2