From: W. Trevor King <wking@drexel.edu>
Date: Wed, 31 Aug 2011 19:08:24 +0000 (-0400)
Subject: Fix BamI sticky end in PstI+BamHI step of I27-synthesis post.
X-Git-Url: http://git.tremily.us/?a=commitdiff_plain;h=d815f72101bc03c4f780a5cadc685c50dc46fbaf;p=blog.git

Fix BamI sticky end in PstI+BamHI step of I27-synthesis post.

Also fix Qiagen linker site link.
---

diff --git a/posts/I27-synthesis.mdwn b/posts/I27-synthesis.mdwn
index 236a996..e5eb42e 100644
--- a/posts/I27-synthesis.mdwn
+++ b/posts/I27-synthesis.mdwn
@@ -204,7 +204,7 @@ The single-SP plasmid, pSP4-1, is split in two parallel reactions.
 #### PstI + BamHI
 
         G...AGATCT-SP-G
-    ACGTC...TCTAGA-SP-CCTAGG
+    ACGTC...TCTAGA-SP-CCTAG
 
 #### PstI + BglII
 
@@ -282,7 +282,7 @@ vector", but I'm not sure why they would need a non-expression vector,
 as they don't reference cross-vector subcloning after inserting their
 I27 monomer into the plasmid.
 
-From the [Qiagen site][pQE30], the section around the linker
+From the [Qiagen site][pQE30-b], the section around the linker
 nucleotides 115 through 203 is:
 
         ,RGS-His epitope__________________. ,BamHI.