From: W. Trevor King <wking@drexel.edu>
Date: Wed, 3 Aug 2011 00:13:52 +0000 (-0400)
Subject: A few more markdown cleanups to I27-synthesis post.
X-Git-Url: http://git.tremily.us/?a=commitdiff_plain;h=7c60bd963e87fadec82aa914a0c507cae48bce87;p=blog.git

A few more markdown cleanups to I27-synthesis post.
---

diff --git a/posts/I27-synthesis.mdwn b/posts/I27-synthesis.mdwn
index 82537c3..236a996 100644
--- a/posts/I27-synthesis.mdwn
+++ b/posts/I27-synthesis.mdwn
@@ -83,7 +83,7 @@ pressured to use mine ;).
 Restriction enzymes
 -------------------
 
-We'll use the following [restriction enzymes][renz]
+We'll use the following [restriction enzymes][renz]:
 
 [BamHI][]
 
@@ -225,7 +225,7 @@ together to form pSP4-2.
     ...................................
 
 where the "other" sequence is the result of the BamHI/BglII splice.
-Expanding the "-SP-" abbreviation around the SP joint:
+Expanding the `-SP-` abbreviation around the SP joint:
 
     ....SP,other_.HindIII.  SP.....
     Leu Met Asp Leu Ser Phe Met Arg
@@ -266,7 +266,7 @@ through 16248.
 This cDNA match generates an amino acid starting with LIKVEK instead
 of the expected LIEVEK, but the LIKVEK version matches amino acids
 12677-12765 in [Q8WZ42][] (canonical titin), and there is a natural
-variant listed for [12679 K->E][var].
+variant listed for [12679 K→E][var].
 
 Interestingly, this sequence contains a PstI site at nucleotides 16220
 through 16225.  None of our other restriction enzymes have sites in
@@ -346,7 +346,7 @@ need to use a third restiction enzyme to insert their I27.
 Since I'm stuck trying to get I27 into either plasmid, let's try and
 work backward from
 
-    Met-Arg-Gly-Ser-(His)₆-Gly-Ser-(I27-Arg-Ser)₇-I27-Cys-Cys
+    Met-Arg-Gly-Ser-(His)₆-Gly-Ser-(I27-Arg-Ser)₇-I27-...-Cys-Cys
 
 #### <a id="BglII-BamHI-joint">BglII/BamHI joint</a>
 
@@ -361,8 +361,6 @@ The BglII/BamHI overlap would produce the expected Arg-Ser joint.
 The beginning of this sequence looks like the start of pQE30's linker,
 so we'll assume the final plasmid was:
 
-  Met-Arg-Gly-Ser-(His)₆-Gly-Ser-(I27-Arg-Ser)₇-I27-Arg-Ser-Cys-Cys
-
     remote ...    ,RGS-His epitope__________________. ,BamHI. I27...
            ... Met Arg Gly Ser His His His His His His Gly Ser Leu Ile ...
     ???    ... ATG AGA GGA TCG CAT CAC CAT CAC CAT CAC GGA TCC CTA ATA ...