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Fix BamI sticky end in PstI+BamHI step of I27-synthesis post.
author
W. Trevor King
<wking@drexel.edu>
Wed, 31 Aug 2011 19:08:24 +0000
(15:08 -0400)
committer
W. Trevor King
<wking@drexel.edu>
Wed, 31 Aug 2011 19:08:24 +0000
(15:08 -0400)
Also fix Qiagen linker site link.
posts/I27-synthesis.mdwn
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diff --git
a/posts/I27-synthesis.mdwn
b/posts/I27-synthesis.mdwn
index 236a996b60401748a9bb6982bb242c39f868e522..e5eb42e53a110dd44f3fd9a4b42ada9a6f66181d 100644
(file)
--- a/
posts/I27-synthesis.mdwn
+++ b/
posts/I27-synthesis.mdwn
@@
-204,7
+204,7
@@
The single-SP plasmid, pSP4-1, is split in two parallel reactions.
#### PstI + BamHI
G...AGATCT-SP-G
- ACGTC...TCTAGA-SP-CCTAG
G
+ ACGTC...TCTAGA-SP-CCTAG
#### PstI + BglII
@@
-282,7
+282,7
@@
vector", but I'm not sure why they would need a non-expression vector,
as they don't reference cross-vector subcloning after inserting their
I27 monomer into the plasmid.
-From the [Qiagen site][pQE30], the section around the linker
+From the [Qiagen site][pQE30
-b
], the section around the linker
nucleotides 115 through 203 is:
,RGS-His epitope__________________. ,BamHI.