3 We can model the I27 multimers as an array of Bell-model unfolders in
4 series with a cantilever. Any unfolded domains also contribute to the
5 tension according to the WLC\index{WLC} tension formula. Completing
6 1000 simulated pulls for each cantilever/pulling-speed/multimer-number
7 combination with our \sawsim\ Monte Carlo simulator yielded the
9 (\cref{fig:cant:sim:v-dep,fig:cant:sim:load-dep,fig:cant:sim:i-dep}).
12 \asyinclude{figures/cantilever-sim/v-dep}
13 \caption{Unfolding force velocity dependence for different
14 cantilevers.\label{fig:cant:sim:v-dep}}
18 \asyinclude{figures/cantilever-sim/loading-rate}
19 \caption{Unfolding force loading rate dependence simulations for
20 different cantilevers.\label{fig:cant:sim:load-dep}}
24 \asyinclude{figures/cantilever-sim/i-dep}
25 \caption{Unfolding force peak index dependence simulations for
26 different cantilevers.\label{fig:cant:sim:i-dep}}
31 %size(6cm,4cm,IgnoreAspect);
32 %scale(Linear, Linear);
36 %graphFile("cantilever/stiffness_force-127_8", xscale, yscale, phard, m30, t=units("127","pN/nm"), dots=true);
37 %graphFile("cantilever/stiffness_force-27_8", xscale, yscale, phard, m8, t=units("27","pN/nm"), dots=true);
42 %label(sLabel("Sawtooth curves ("+units("v = 1","$\mu$m/s")+", 8 domains)"), point(N),N);
43 %xaxis(sLabel("Distance (nm)"),BottomTop,LeftTicks);
44 %yaxis(sLabel("Tension (pN)"),Left,RightTicks);
46 %picture q=secondaryY(new void(picture pic) {
47 % scale(pic,Linear,Linear);
48 % graphFile(pic, "cantilever/stiffness_stiffness-127_8", xscale, 1e3, pmed, m30, dots=true);
49 % graphFile(pic, "cantilever/stiffness_stiffness-27_8", xscale, 1e3, pmed, m8, dots=true);
50 % ylimits(pic, 0, 130);
51 % yaxis(pic, sLabel("Stiffness (pN/nm)"),Right, LeftTicks);
55 %add(legend(),point(E)+(24pt,0),20E,UnFill);